Simple and inexpensive method for cryopreservation of fish sperm combining straw and powdered dry ice.

نویسندگان

  • George Shigueki Yasui
  • Lenin Arias-Rodriguez
  • Takafumi Fujimoto
  • Katsutoshi Arai
چکیده

Here, we propose a simple and inexpensive method for fish sperm cryopreservation. Sperm samples of the loach Misgurnus anguillicaudatus (Teleostei: Cobitidae) were diluted 7-fold by an extender containing 63.5 mM NaCl, 114 mM KCl, 20 mM Tris and 10% methanol. The cryogenic straws were placed in three kinds of self-made tubes which diameter was changed by commercially available materials and then immersed into powdered dry ice for 2 min and plunged into liquid nitrogen. This procedure resulted in a cooling rate at -421.4 +/- 119.84 (control), -55.8 +/- 4.32 (tube 1), -40.2 +/- 3.43 (tube 2) and -33.3 +/- 2.09 C/min (tube 3). In the slowest cooling rate by the tube 3, total motility (72 +/- 3 %), duration (146 +/- 12 s) and hatching rates (29 +/- 04 %) were higher than those by other rates. Progressive motility (83 +/- 5 %) did not differ significantly from fresh samples.

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عنوان ژورنال:
  • Cryo letters

دوره 29 5  شماره 

صفحات  -

تاریخ انتشار 2008